Journal: BMC Complementary and Alternative Medicine

Article Title: Total phenolic, flavonoid contents, in-vitro antioxidant activities and hepatoprotective effect of aqueous leaf extract of Atalantia ceylanica

doi: 10.1186/1472-6882-14-395

Figure Lengend Snippet: Percentage enzyme leakage of porcine liver slices after 2 hour exposure to medium (negative control), ethanol (5 M) and different concentrations of A. ceylanica (400, 1000, 2000 μg/ml) with ethanol (5 M). Each value represents mean + SD (n =3). * p <0.05 when compared to ethanol (5 M) treated sample for the corresponding enzyme assayed. AST = Aspartate transaminase, ALT = Alanine transaminase, LDH = Lactate dehydrogenase.

Article Snippet: Lactate dehydrogenase (LDH) enzyme assay kit was purchased from DiaSys (Alte Strasse 9, 65558, Holzheim, Germany).

Techniques: Negative Control

Journal: Frontiers in Microbiology

Article Title: Global Profiling of Lysine Acetylation in Borrelia burgdorferi B31 Reveals Its Role in Central Metabolism

doi: 10.3389/fmicb.2018.02036

Figure Lengend Snippet: Metabolism is affected by acetylation. (A) Heat-map showing the number of acetylated lysines at ML versus S phase in wild-type for all proteins involved in metabolism or transport. (B) Glycolysis and glycerol pathways with the number of sites acetylated at each growth phase indicated. Proteins that were more acetylated at S phase than ML phase were indicated in yellow. (C) Domain schematics of selected proteins and localization of acetylated lysines. (D) 3D structure of GAPDH and LDH proteins showing the potential impact of adding an acetyl group to a lysine residue. (C,D) Domains (active site, in red/pink; NAD or NADH pocket, in blue) were based on UniProt and Prosite database. Acetylated lysine were indicated in light gray (only at ML phase), in dark gray (at both growth phase), in orange (only at S phase).

Article Snippet: LDH and GAPDH were overexpressed and purified by GenScript.

Techniques: Residue

Journal: Frontiers in Microbiology

Article Title: Global Profiling of Lysine Acetylation in Borrelia burgdorferi B31 Reveals Its Role in Central Metabolism

doi: 10.3389/fmicb.2018.02036

Figure Lengend Snippet: Acetylome in the Δ ackA , Δ pta and their complemented strains. (A) Acetate/Mevalonate pathway in B. burgdorferi . (B) Wild-type (lane a), Δ ackA (lane b), Δ pta (lane d), ackA complemented (lane c), and pta complemented (lane e) strains were grown in BSK-II with 500 μM of mevalonolactone. Cell lysates were analyzed by SDS-PAGE, coomassie blue staining and immunoblotting by probing with AckA, Pta, Acat, GAPDH, Eno, LDH, and FlaB antigen-specific antisera. Uncropped gels are displayed in Supplementary Figure . (C) Number of unique acetylated peptides in wild-type, ackA , Δ pta and their complemented strains.

Article Snippet: LDH and GAPDH were overexpressed and purified by GenScript.

Techniques: SDS Page, Staining, Western Blot

Journal: Frontiers in Microbiology

Article Title: Global Profiling of Lysine Acetylation in Borrelia burgdorferi B31 Reveals Its Role in Central Metabolism

doi: 10.3389/fmicb.2018.02036

Figure Lengend Snippet: Lysine acetylation directly affected the enzymatic activities of proteins involved in glycolysis. Enzyme activities of GAPDH (A) , LDH (B) , and Eno (C) were measured from crude extracts of the wild-type and the Δ ackA mutant at ML and S phase and normalized with total protein (see the section “Materials and Methods”). Effect of acetylation by Ac-P on enzymatic activity assayed in the presence or absence of Ac-P on GAPDH (D) , LDH (E) or Eno (F) purified proteins. For LDH and GAPDH, one unit is the amount of enzyme able to generate 1 μmole of NADH per minute (nmole/min/μL). For Eno, one milliunit is the amount of enzyme able to generate 1 nmole of H 2 O 2 per minute (nmole/min/μL). Results are expressed at nmole/min/mg of total protein or μg of purified protein. Results reported are the average of at least three independent experiments. Data represent means ± standard deviation. An asterisk indicates a significant difference using a one-way ANOVA where p < 0.05.

Article Snippet: LDH and GAPDH were overexpressed and purified by GenScript.

Techniques: Mutagenesis, Activity Assay, Purification, Standard Deviation

Journal: BMC Evolutionary Biology

Article Title: D- and L-lactate dehydrogenases during invertebrate evolution

doi: 10.1186/1471-2148-8-268

Figure Lengend Snippet: List of sequences included in the phylogenetic analyses.

Article Snippet: LDH-B , Gallus gallus , chicken , [Swiss-Prot: P00337 ] , Tsoi et al. 1998 unpublished.

Techniques:

Journal: Heliyon

Article Title: Analysis of immunological and biochemical parameters after booster dose vaccination using protein-based and inactivated virus vaccine for safety

doi: 10.1016/j.heliyon.2024.e40124

Figure Lengend Snippet: A) Changes in LDH enzyme, B) Changes in CPK enzyme, C) Changes in CK-MB enzyme, 14 days after vaccination with the RCP and sinopharm/BBIBP-CorV.COVID-19 vaccine.

Article Snippet: A) Changes in LDH enzyme, B) Changes in CPK enzyme, C) Changes in CK-MB enzyme, 14 days after vaccination with the RCP and sinopharm/BBIBP-CorV.COVID-19 vaccine.

Techniques: